The Anti-MEKK1 antibody from MyBioSource.com is a Rabbit Polyclonal antibody to MAP3K1, MAPKKK1, MEKK, and MEKK1. This antibody recognizes Human, Mouse, and Rat antigen. The Anti-MEKK1 antibody has been validated for the following applications: Immunohistochemistry, Immunohistochemistry - fixed, and Western Blot.
Description
Description: Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 1(MAP3K1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: MAP3K1(Mitogen-activated protein kinase kinase kinase 1), also known as MEKK1, MAPKKK1, MEK KINASE or MAP/ERK KINASE KINASE 1, is an enzyme that in humans is encoded by the MAP3K1 gene. Vinik et al.(1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse Map3k1 gene and the human MAP3K1 homolog. Using these allele-specific polymorphisms, they mapped the Map3k1 gene in an intersubspecific backcross to mouse chromosome 13. They mapped the human MAP3K1 gene to chromosome 5 by somatic cell hybrid analysis. By assaying transfected COS-1 cells, Xia et al.(1998) showed that human MEKK1 activated JNK1(MAPK8) robustly and p38-alpha(MAPK14) less efficiently, but it had only a marginal effect on ERK2(MAPK1). MEKK1 directly and specifically interacted with JNKK1(MAP2K4) and activated JNKK1 in cells and in vitro. Phosphorylation of JNKK1 by MEKK1 disrupted their interaction. MEKK1 and JNK1 competed for binding to JNKK1. Xia et al.(1998) concluded that JNKK1 is the preferred MEKK1 substrate